Beckman HPLC procedure
buffer A, buffer B and ACN, make sure not empty.
blank solution (H2O) vial not empty. Check makeup buffer vial
not empty and is in 85 position.
32 Karat software. Click “Beckman” icon, Click “OK” for instrumental wizard.
- Go to
Control – Direct Control. Set B as 100% Set flow rate 1ml/min &
duration time as 1 min.
on Lamp by clicking the lamp icon at the right side, and wash column by
clicking the syringe icon in the middle. This only needs to be done once
Start up program
on blue arrow in the menu bar.
down sample ID (this could be the same for a series of runs)
method, choose “thiolDNA—startup”.
down file name (this must be different for each run)
vial number, type down the number the blank solution vials sits in.
injection volume as 5 uL.
until this step is over (Red stop sign on toolbar menu will fade)
sure makeup buffer is not empty and is on position 85.
on blue arrow to start. Choose “thiolDNA-injection” methods. Vial number
should be the number your sample is sitting in. And volume is the exact
volume of your sample.
20 minutes after, collect the sample from the drain.
- If you
have more samples to inject, just wait until the program finishes, then go
to step 17; otherwise stop the program by clicking the stop sign and set
B% to 10% at direct control window.
on blue arrow and choose “thiolDNA-finalclean” method; follow step 9-11.
and lamps will be turned off automatically.
**When changing flow rate or
solution percentage manually, always give at least 1 min duration time.